Sequence homology of androgen-regulated epididymal proteins
نویسنده
چکیده
a 5\m='\-incompleteopen reading frame of 525 base pairs. The 3\m='\-untranslatedregion of 946 base pairs contains a repetitive DNA element of the rodent B1 family just between two canonical polyadenylation signals AATAAA, upstream of the poly(A) track. The deduced amino acid sequence for the Mr 24 000 proteins reveals significant homologies of 66\m=.\6,66, 65\m=.\2,63\m=.\1 and 64\m=.\5%with mouse, rat, man, bull and rabbit cloned selenium\x=req-\ dependent glutathione peroxidases, respectively. The present results emphasize previous studies performed in numerous laboratories suggesting that the major protective system against oxidative damage in mouse spermatozoa could be an enzyme similar to glutathione peroxidase.
منابع مشابه
A mouse cDNA sequence for epididymal androgen-regulated proteins related to glutathione peroxidase.
A cDNA was isolated from a murine epididymal cDNA library REFERENCES in Xgtll by differential hybridization. This cDNA codes for , „, ,. , .. D ,. „ , f . . . . . _ c IT1 / i n n n . , _ .. . ? , , . . . . , , ,s m '• Ghyselinck.N.B., Jimenez.C, Lefrancois.A.M. and DufaureJ.P. (1990)/ 24kDa androgen-regulated epididymal secretory proteins (1). The M^ gru^r 4 5-12 sequence is presented below, al...
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The retinoic acid binding activity in the lumen of the rat epididymis (Ong, D., and Chytil, F. (1988) Arch. Biochem. Biophys. 267, 474-478) has been purified to homogeneity. The protein exists in two forms, one form having an additional three amino acids at the amino terminus. The amino acid sequence of the protein was determined to 20 amino acids and proved to be identical to that of the major...
متن کاملGenetic evidence for an androgen-regulated epididymal secretory glutathione peroxidase whose transcript does not contain a selenocysteine codon.
Epididymal glutathione peroxidase (GPX) has been suggested as a major factor in combating loss of fertility of spermatozoa due to lipid peroxidation. We report here the isolation and sequence of putative GPX cDNAs from rat (Rattus rattus) and cynomolgus-monkey (Macaca fascicularis) epididymis, which exhibit marked sequence identity with known GPXs. In both species the cDNAs encode predicted pre...
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The epididymis is the site for the transport, maturation, and storage of spermatozoa. Regulation of epididymal structure and function is highly dependent on the ipsilateral testis. At the molecular level, however, few studies have been undertaken to determine which genes are expressed in the epididymis under testicular regulation. The goal of this study was to identify genes for which expressio...
متن کاملSperm-associated antigen 11A is expressed exclusively in the principal cells of the mouse caput epididymis in an androgen-dependent manner
BACKGROUND Epididymal sperm maturation occurs via interactions between sperm and proteins secreted by the epididymal epithelium. Although this is an important process, the genes that encode the involved proteins remain largely uncharacterized. Previous studies have demonstrated that the genes involved in sperm maturation are regulated by androgen. Spag11a is an epididymal gene that is influence...
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